NMR Spectroscopy

Nuclear Magnetic Resonance (Bruker NMR – Ultrashiled – 400 mHz):

1D – Proton, Carbon and Phosphorus STD, Water LOGSY
2D – COSY, TOCSY, ROESY, NOESY, DOSY; DOSY-TOCSY (3D), RDC, T1, T2, T1p relaxation
2D HMQC, HMBC & HSQC analysis for H-C and H-P correlations, peptides and metabolites such as ATP, ADP and NADP

What is NMR spectroscopy used for?

Nuclear magnetic resonance spectroscopy examines how certain atomic nuclei respond in a magnetic field. It provides information about molecular environments and can support structural characterization, identity investigations, and comparison of related samples.

How do 1D and 2D NMR experiments differ?

A one-dimensional experiment presents signals along one frequency axis. Two-dimensional experiments reveal selected relationships between signals, helping analysts investigate connectivity or other molecular features. The appropriate experiment depends on the structure question and sample properties.

What should you share before submitting an NMR sample?

Provide the expected structure, sample composition, available quantity, solubility information and specific objective. Mention whether you need an identity check, structural investigation or another defined analysis. The laboratory should confirm solvent, quantity and experiment requirements before submission. For the role of characterized materials in routine quality control, read our guide to reference and working standards

Can NMR alone establish complete pharmaceutical quality?

No. Structural information is one part of a testing strategy. Other quality attributes may require complementary methods and a product-specific specification.