At Tamilnadu Test House, we are committed to excellence in analytical testing and regulatory compliance. As many of you are aware, we are already licensed by CDSCO under FORM-37 for pharmaceutical and cosmetic product analysis under COS-23, alongside our NABL accreditation.
Ayush Testing
We are thrilled to announce that Tamilnadu Test House has now received approval under FORM-48 to conduct tests and analyses for Ayurvedic, Siddha, and Unani drugs, as well as raw materials used in their manufacturing. This milestone allows us to extend our expertise to AYUSH licensees, further strengthening quality assurance in traditional medicine.
Our Comprehensive AYUSH Testing Services
We now offer a range of specialized analyses to support the quality and compliance of Ayurvedic, Siddha, and Unani products, including:
- Stability Studies
- HPTLC Fingerprinting & Quantification
- Herbal cosmetics analysis
- Pesticide Residue Analysis
- Organochlorine pesticides Analysis
- Organophosphorus pesticides Analysis
- Pyrethroids Analysis
- Aflatoxin Detection
- Residual Solvent Testing
- Microbiological Testing
- Herb & Plant Extract Identification
- Heavy metal analysis
- Physio Chemical analysis.
For any inquiries or collaborations, feel free to reach out to us. Let’s work together to uphold excellence in AYUSH product testing!
Tamilnadu Test House offers HPTLC Testing services in the below areas for identification and Quantification of the components. HPTLC is a sophisticated instrument which is used for comparing several samples in parallel, checking for adulteration, Purity analysis, and quantifying marker compounds.
- Botanical industry for fingerprinting, Identification and quantification of marker compounds, adulterant and falsification.
- Pharmaceutical applications for identification and impurity test.
- Food and cosmetic industry – Aflatoxins, Lipids, Carbohydrates, Food Colors, pigments, other additives
- Environmental analysis – Pesticides, PAH’s
- Forensics / toxicology.
Stability Testing for AYUSH Products
Stability testing is crucial for AYUSH (Ayurveda, Yoga & Naturopathy, Unani, Siddha, and Homeopathy) products to ensure their safety, efficacy, and quality over their shelf life. This process helps determine how environmental factors such as temperature, humidity, and light affect the product.
Tamilnadu test House provides the stability study services to determine the shelf life and storage conditions.
Tamilnadu conducts the stability study as below:
| Study | Storage condition | Duration |
|---|---|---|
| Long-Term Stability | 25°C ± 2°C, 60% RH ± 5% RH | Minimum 12 months |
| Accelerated Stability | 40°C ± 2°C, 75% RH ± 5% RH | 6 months |
| Intermediate Stability | 30°C ± 2°C, 65% RH ± 5% | Case-specific |
Parameters for Stability Testing
Category Tests Performed
Physical: Appearance, color, texture, phase separation, weight variation
Chemical: Active ingredient stability, pH, moisture content, degradation products
Microbial: Total viable count, yeast & mold, pathogenic bacteria (E. coli, Salmonella, etc.)
Therapeutic: Potency of active ingredients (e.g., alkaloids, Flavonoids)
Packaging: Interaction with packaging materials, leakage, integrity
Guidelines & Regulatory Framework
1. AYUSH Ministry Guidelines (India)
2. Schedule T of the Drugs & Cosmetics Act, 1940 (for Good Manufacturing Practices – GMP)
3. WHO Guidelines for Stability Testing of Herbal Medicines
4. ICH Guidelines (where applicable)
Stability testing is essential for AYUSH product standardization and global acceptance. TNTH expert team ensure the consumer safety and product reliability with proper scientific validation which gives higher credibility and regulatory compliance to the traditional medicines.
Herbal Drugs
- HPTLC fingerprinting of herbal drugs used in gemmotherapy
- Rapid chemotaxonomic discrimination of Clerodendrum species
- Analysis of innovative plant extracts
- Pharmacokinetics of berberine from Pushyanuga Churna
- Simplified analysis of purity of ginkgo products
- HPTLC-UV fingerprints of Gelsemium elegans and koumine contents determined by densitometry compared to UPLC-MS/MS
- Comprehensive HPTLC fingerprinting for the quality control of Angelica gigas root
- Qualitative and quantitative HPTLC analysis of licorice root
- Comparison of conventional TLC and HPTLC for identity testing of herbal medicinal extracts
- Adulteration of St. John’s Wort Products (Herbal Medicine Manufacturer)
- The unique merits of HPTLC image analysis for quality control of herbal medicines
- Marker compounds in Java tea characterized by HPTLC
- Screening of three PDE5-Inhibitors and eight of their analogs in lifestyle products
- Bioassay-guided isolation of plant antibiotics
- Quantification of alkaloids in Sceletium tortuosum
- Identification of acetylcholinesterase inhibitors from Galbanum
- Planar-chromatographic fingerprint of German propolis
- Rapid structure confirmation and quantitation by HPTLC-NMR
- Bioautographic HPTLC assays for screening of Gabonese medicinal plants used against Diabetes mellitus
- Identification of polyphenolic compounds in Rheum officinale Baill. by TLC-MS-coupling
- Simultaneous analysis of temephos and fenitrothion in green tea
- TLC/HPTLC fingerprinting of herbal essential oil followed by liquid chromatography hyphenated with the TLC-MS Interface
- Quality control of Traditional Chinese Medicines by HPTLC
- Separation of common plant triterpenoids by HPTLC
- A comparison between HPLC and HPTLC for the separation and quantification of boswellic acids in Boswellia serrata extracts
- Screening of unknown plant extracts by planar chromatography
- HPTLC-identification of Hoodia gordonii, a popular ingredient of botanical slimming products
- Validation of HPTLC methods for the identification of botanicals
Food
- Characterization of E472 food emulsifiers by HPTLC fingerprints
- Comparison of yeast estrogen screening on HPTLC and in microtiter plates
- Analysis of neonicotinoids in honey by complementary use of Planar Chromatography – HPLC and Mass Spectrometry
- Screening of weight loss products for deliberately added undeclared synthetic drugs
- Lovastatin and citrinin in red yeast rice products
- Screening of steroids as adulterants in food supplements
- Fast determination of benzoic acid in food
- Fast determination of benzoic acid in food
- HPTLC quantification of cocoa ingredients and their changes during different chocolate manufacturing steps
- Quantification of Bitter Acids in Hops
- Quantification of steviol glycosides and steviol/isosteviol
- Screening for ricinoleic acid as marker for Secale cornutum impurities in rye
- Rapid screening for ergot alkaloids in rye flour by planar solid phase extraction (pSPE)
- Quantification of wax ester content in escolar
- Determination of the hemolytic activity of saponins by an HPTLCblood gelatin test
- Identification of herbal slimming drugs and screening for adulteration by HPTLC
- Determination of lactose in foodstuff
- Modern direct bioautography of endocrine active compounds
- HPTLC-UV/MS of caffeine in energy drinks
- Anthocyanes in food and animal feed by HPTLC-Vis-(EDA-)MS
- Solid phase extraction as clean-up for pesticide residue analysis of tea samples using planar chromatographic developing techniques
- Quantitative determination of steviol glycosides (Stevia sweetener)
- TLC screening for the detection of Robusta admixtures to Arabica coffee
- The fingerprint of biopolymers (polysaccharides)
- Fast quantification of 5-hydroxymethylfurfural in honey
- Planar solid phase extraction – a new clean-up concept in residue analysis of pesticides
- Determination of enrofloxacin and ciprofloxacin in milk by direct bioautography detection
- Determination of the glycoalkaloids -solanine and -chaconine in potatoes at different steps of potato processing
- Analysis of water-soluble food dyes
- Determination of unauthorized fat-soluble azo dyes in spices by HPTLC
- HPTLC determination of illegal dyes in chili, paprika und curry
- Quantification of piperine in black pepper fruit (Piper nigrum L.) and test for minimum content (MCT) of piperine (> 3% Ph. Eur.)
- Limit test for adulteration of black pepper fruit (Piper nigrum L.) with papaya seed (Carica papaya L.)
- Identification of Tangerine Peel (Citrus reticulata Blanco)
Biotechnology
- Characterization of the invertase activity and identification of botanical substrates by densitometry and MALDI-TOF MS
- Dextrin profiles of starch digested with different amylases
- Analysis of insulin samples from different species by HPTLC-MS
- Use of Planar Chromatography for the analysis of peptides from tryptic protein digest
Cosmetics
- Screening for natural cosmetic preservatives by HPTLC-EDA
- Validated method for fast quantification of glycine in cosmetics
- AMD analysis and determination of biocides in lens cleaning fluid
- Quality control of cosmetic products by HPTLC
- Quantification of xanthones in mangosteen fruit hull extracts
- HPTLC bioautographic assay for tyrosinase inhibitors in plant extracts
- Screening method to study the reactivity of cosmetic UV filters on skin proteins
- Detection and determination of caffeine, taurine and arginine in shampoos
- Quantification and side component analysis of the cosmetic active tiliroside using planar chromatography
- Determination of aloe vera gel in cosmetics
- HPTLC fingerprint of Edelweiss plants and extracts used as ingredients in cosmeceuticals
- Detection of UV filters in cosmetic products (sunscreen) by HPTLC and confirmation by HPTLC-MS
- HPTLC method for determination of ceramides from human skin
Pharmaceutical
- TLC and HPTLC-MS in the manufacturing of clinical API batches
- Degradation profiling of cefixime and azithromycin (antibiotics).
- Cleaning validation at API production units
- Simultaneous determination of pioglitazone, metformine and glimepiride in pharmaceutical preparations
- Rapid test for content uniformity of Coenzyme Q10 in soft gel capsules by HPTLC
- Cleaning validation using HPTLC
- TLC/HPTLC-ELSD-MS coupling
- Validated determination of secoisolariciresinol diglucoside in flaxseed by HPTLC
- Screening for bioactive natural products in sponges
- Use of HPTLC as a problem solving technique in pharmaceutical analysis
- Identification and quantification of amino acids in peptides
- Fast identification of unknown impurities by HPTLC/MS
- Rapid content uniformity test of 6 batches of coenzyme Q10 in soft gel capsules by HPTLC
- Determination of Metronidazole in pharmaceutical dosage form by HPTLC
- Detection of Impurities in Bupropion Hydrochloride by HPTLC
Chemical
- Polycyclic aromatic hydrocarbons in road surfaces
- The CAMAG TLC-MS Interface 2 in practice
- Determination of PAHs in toys by HPTLC
- Determination of additives in plastic foils
- Determination of SARA (Saturates, Aromatics, Resins and Asphaltenes) in bitumen by HPTLC
Environmental
- Evolution of plant defense compounds in the plantlitter continuum
- Effect-directed analysis with HPTLC for water and environmental samples
- Effect-directed analysis of a water sample
- New screening concept for pesticide residue analysis in fruit and vegetables – HTpSPE-HRMS
- HPTLC-MS combined with H/D exchange for the identification of substances in environmental analysis
- Effect-directed analysis of environmental samples
- Drinking water treatment – Identification of reaction by-products of 4- and 5-methyl1H-benzotriazole formed during ozonation
- HPLC-MS or simply HPTLC for analysis of sucralose in water?
- 1H-Benzotriazole and tolyl triazole in the aquatic environment
Other
- Characterization of the invertase activity and identification of botanical substrates by densitometry and MALDI-TOF MS
- HPTLC-based effect-directed workflow in drug discovery
- Optimized HPTLC-MS method for identification of constituents in broad-leaved dock
- Comparison of different derivatization techniques
- HPTLC for the quantitation of nicotine in liquids for electronic cigarettes
- CAMAG Derivatizer – New spraying device for the automated derivatization of TLC plates
- Quantification of nicotine in liquids for electronic cigarettes
- Vision CATS 2.0 – Our new software for qualitative and quantitative HPTLC analysis
- Analysis of plant glycosylceramides by automated multiple development
- Introducing the new generation software – visionCATS
- Introduction of special HPTLC and TLC plates for coupling with mass spectrometry
- HPTLC-MS analysis using a novel compact single quadrupole mass spectrometer
- Optimization of an AMD2 method for determination of stratum corneum lipids
- Validated HPTLC method for skin lipids
- The new TLC-MS Interface
- Use of reversed phase (RP)-modified pre-coated plates
Tamilnadu Test House Private Limited Routinely
Undertakes Contract Lab Testing
PHARMACEUTICALS
FOOD TESTING
WATER & ENVIRONMENT
POLYMER TESTING
PERSONAL CARE & COSMETICS
BIO TECHNOLOGY
