What are the methods of measuring refractive index?

What Are The Methods Of Measuring Refractive Index?

The way light moves through a substance is determined by its refractive index. Various techniques are used to measure the refractive index, depending on the material and the degree of accuracy needed. As one of the best testing companies in Chennai, we employ refractive index measurements to assess the purity and concentration of gases, liquids, semi-liquids, and solid samples. Here, we may learn the most common approaches, including:

Abbe Refractometer


The refractometer determines the critical angle by monitoring the total internal reflection at the prism contact as light travels through a sample.
It is typically applied to liquids and transparent materials.

Snell’s Law Method

Refractive index testing labs in India Use Snell’s Law to measure angles of incidence and refraction with a goniometer or optical microscope

Critical Angle Refractometry: 

This applies to transparent materials
Measures the angle of total internal reflection between a sample and a reference medium
Applicable to liquids and thin films

Michelson interferometer

Determines gas and thin film refractive indices using interference patterns.
High precision demands controlled environmental conditions.

Brewster’s Angle Method

The angle at which light is perfectly polarized upon reflection is calculated using a formula.
Ideal for thin films and dielectric materials.

Spectroscopic Ellipsometry

Measures polarization shifts in reflected light to calculate refractive index and film thickness.
Suitable for thin films and coatings.

The Sellmeier and Cauchy’s Equations

Empirical models that link wavelength and refractive index
For precise fitting, experimental data from several wavelengths is needed.

Optical Coherence Tomography (OCT):

This technique uses low-coherence interferometry to assess changes in the refractive index of biological tissues. Frequently used in imaging medicine.

Pulfrich Refractometers:

This gadget accurately detects the refractive index of liquids by measuring the deviation of a light beam as it passes through a prism containing the sample.

Fiber Optic Refractometry:

This method measures the refractive index by utilizing the interaction of evanescent waves in optical fibers
Common in applications involving biosensing

Every technique has benefits and is selected according to the type of material, required level of accuracy, and experimental setup.

What It Is and Its Role in Industry Definition of Disinfection

Various industry categories take the help of refractive index measurements to characterize different samples:

Food and beverage: Helps in measuring the sugar content of soft drinks

Chemical: Determines the freezing point, acid/base concentration and the presence of organic solvent and inorganic salt

Pharma: Checks for the presence of hydrogen peroxide and methanol

To elaborate, below are some of the essential commodities that take the help of refractive measurement to measure their purity:

For Flavour and the essential oil industry

  • Perfumes
  • Skin cleansers
  • Lotions
  • Lemon, lime, orange
  • Palm
  • Creams
  • Waxes
  • Natural oils
  • Sandalwood


Food and beverage

  • Seed oils
  • Soybean oils
  • Candy
  • Syrups
  • Dairy products
  • Edible oils
  • Coffee extracts
  • HRCS
  • Juices
  • Juice concentrates
  • Fruit products
  • Confections
  • Starch
  • Milk
  • Hydrolysis products
  • Vegetable products
  • Teas
  • Sodas
  • Sauces
  • Soups
  • Jams
  • Jellies
  • Soft drink


Sugar industry

  • Cane sugar
  • Beet sugar
  • Invert sugar
  • Liquid sugar
  • Confectionery sugar
  • Brown sugar


Petroleum industry

  • Oil based paints
  • Fine chemicals
  • Fluranes
  • PET
  • Polymers
  • Resins
  • Solvents
  • Plastics

At Tamilnadu Test House, one of the best-rated Refractive Index testing lab, we use the latest refractometers and technologies to determine the purity of your products. Our assured results help you establish yourself as a trusted brand in the market while gaining you a competitive edge. Contact us for your requirements.